The Epstein-Barr virus lytic cycle activator Zta interacts with methylated ZRE in the promoter of host target gene egr1

This was my first paper, from my master’s work in the Sinclair lab at Sussex. This investigated the binding of the EBV transactivating Zta protein to the host human EGR1 gene. We used EMSAs to identify which of the prospective sites were bound most readily, factoring in this protein’s ability to preferentially bind to methylated response elements.